FIGURE

Fig 2

ID

ZDB-FIG-220227-21

Publication

Berger et al., 2022 - Genetic dissection of novel myopathy models reveals a role of CapZα and Leiomodin 3 during myofibril elongation

Other Figures

All Figure Page

Back to All Figure Page

Fig 2

Lmod3-deficiency leads to weakening of the trunk and head musculature.

(A) Peak active force of single-twitch contractions generated by 6-dpf-old individual lmod3^sa13018 larvae was significantly weaker compared to their siblings. Siblings generated a maximal active force of 0.82 ± 0.01 mN, compared to and 0.19 ± 0.03 mN generated by lmod3^sa13018 homozygotes (n = 4). (B) Z-stack projections of ventral views depict the cephalic musculature marked by GFP driven by the Tg(−503unc:GFP) transgene. In contrast to 4-dpf-old siblings, a gap between the two hyohyoideus (hh) muscles was formed in the lower jaw of lmod3^sa13018 homozygotes (asterix). (C) At 7dpf, focus stacks of Alcian Blue cartilage stains revealed a widened jaw within lmod3^sa13018 mutants. (D) The angle formed by the ceratohyal cartilages (dotted lines) was significantly wider in lmod3^sa13018 homozygotes compared to siblings (87 ± 2° and 65 ± 1°, respectively. n = 6). Data are presented as mean ± SEM; *** P < 0.001 calculated by Student’s t test. Scale bar sizes are 200 μm.

Expression Data

Gene:	EGFP
Fish:	pc11Tg lmod3^{sa13018/sa13018}; pc11Tg
Anatomical Term:	hyohyoideus
Stage:	Day 4

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	lmod3^{sa13018/sa13018} (TU) lmod3^{sa13018/sa13018}; pc11Tg
Observed In:	ceratohyal cartilage left side ceratohyal cartilage right side hyohyoideus left side hyohyoideus right side trunk musculature skeletal muscle contraction
Stage Range:	Day 4 to Days 7-13

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ PLoS Genet.