Fig. 4.

PFOS exposure during colitis leads to increased systemic levels and intestinal permeability in mice. (A) Violin plots represent normalized PFOS levels detected in serum (middle) and liver (right) at day 3 after TNBS administration, following the experimental outline shown in the scheme (left). Values have been calculated as fold change of the average concentration detected in the PFOS group of each experiment. Values for vehicle and TNBS groups were not detected (nd). n=8-9, three experiments. (B) Intestinal permeability to 4 kDa FITC-Dextran measured in serum at 4 h after oral gavage at day 1 following TNBS administration and PFOS (5 mg/kg/total dose), following the experimental outline shown in the scheme. Data shown in violin plots relative to the average of the values of the vehicle group in each experiment. n=7-9, three experiments. (C) Mice received a single dose of TNBS (1%, in 50% ethanol, intrarectally administered), four doses of PFOS (10 mg/kg/total dose, orally gavaged) and 500 µg per dose of anti-Ly6G monoclonal antibody every other day, following the experimental outline shown in the scheme. Intestinal permeability measured in serum at 4 h after oral gavage at day 3 post-TNBS administration. Data shown in violin plots are relative to the average of the values of the vehicle group in each experiment. n=5-7, three experiments. The black line represents the median. N.S., not significant; *P<0.05, **P<0.01, ***P<0.001. One-way ANOVA with Fisher's LSD test.