FIGURE

Figure 2

ID
ZDB-FIG-211120-40
Publication
Ding et al., 2021 - The chromatin-remodeling enzyme Smarca5 regulates erythrocyte aggregation via Keap1-Nrf2 signaling
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Figure 2

The blood clots are formed by RBC aggregation.

(A) The snapshot of Tg (gata1:dsRed) in smarca5zko1049a and their siblings from 36 hpf to 2 dpf. The yellow rectangular boxes in CHT show the formed blood clots smarca5zko1049a. (B) The bright-field of parabiosis at 2 dpf between smarca5zko1049a and their siblings. The amplification region in the red rectangular box in CHT shows the blood clots (indicated by arrow heads) in smarca5zko1049a and their siblings. (C) The confocal imaging of parabiosis generated between smarca5zko1049a and their siblings with Tg (gata1:GFP) and Tg (gata1:dsRed) background, respectively. The magnification in CHT shows the aggregation of gata1:GFP+ cells (indicated by arrow heads) in the caudal vein plexus. (D) The confocal imaging of Tg (CD41:GFP) in smarca5zko1049a and their siblings at 2 dpf. The magnification in the yellow rectangular box in CHT shows the blood clots and the distribution of CD41:GFP+ cells. (E) Drugs used to examine whether the blood clots in smarca5zko1049a can be alleviated. (F) The bright-field of tail region in smarca5zko1049a at 2 dpf in control group and with argatroban treatment. The blood clots are indicated by arrow heads. (G) The quantification of blood clots phenotype in (F). Data are mean ± s.d. (G). Asterisk presents statistical significance (**p< 0.01). p Values were calculated by two-tailed unpaired Student’s t-test.

Expression Data
Genes:
Fish:
Anatomical Terms:
Stage Range: Prim-25 to High-pec

Expression Detail
Antibody Labeling
Phenotype Data
Fish:
Condition:
Observed In:
Stage Range: Prim-25 to Long-pec

Phenotype Detail
Acknowledgments
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