ZFIN ID: ZDB-FIG-210819-23
Zhao et al., 2020 - Twist3 is required for dedifferentiation during extraocular muscle regeneration in adult zebrafish. PLoS One   15:e0231963 Full text @ PLoS One
ADDITIONAL FIGURES
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage: Day 5

Fig. S2 Tg(α-actin::EGFP) embryos that were injected with twist 1a/b, 2, or 3 MO at the one- to four-cell state demonstrated EOM formation at 5 dpf from dorsal (B-D), lateral (B'-D'), Ventral (B''-D''), and phenotype (B‴-D‴) compared with control embryos (A-A‴). SO: Superior Oblique, SR: Superior Rectus, LR: Lateral Rectus, IO: Inferior Oblique, IR: Inferior Rectus. Asterisk: undeveloped jaw muscle, red arrow: incorrectly inserted EOM, scale bar: 100μm.

Gene Expression Details No data available
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
zf13Tg + MO2-twist1a + MO4-twist1b standard conditions Day 5 eye decreased size, abnormal
Day 5 mandibular muscle morphology, abnormal
Day 5 pericardium edematous, abnormal
Day 5 post-vent region morphology, abnormal
Day 5 trunk morphology, abnormal
zf13Tg + MO2-twist2 standard conditions Day 5 extraocular musculature position, abnormal
Day 5 eye decreased size, abnormal
Day 5 head muscle morphology, abnormal
Day 5 pericardium edematous, abnormal
Day 5 post-vent region morphology, abnormal
Day 5 trunk morphology, abnormal
zf13Tg + MO3-twist3 standard conditions Day 5 dorsal oblique extraocular muscle morphology, normal
Day 5 dorsal rectus morphology, normal
Day 5 eye decreased size, abnormal
Day 5 eye protruding, abnormal
Day 5 lateral rectus morphology, normal
Day 5 pericardium edematous, abnormal
Day 5 post-vent region morphology, abnormal
Day 5 trunk morphology, abnormal
Acknowledgments:
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ PLoS One