Inhibitory efferent projections to the lateral line in zebrafish larvae. (A) Overview of the inhibitory efferent projections in a Tg(dmrt3a:GAL4;UAS:RFP) 5 dpf larvae. Arrowheads indicate sites of neuromast innervation. Note that this is not the original confocal image, but a modified version to show the inhibitory efferent projections only; for the original image, please see Supplementary Figure 1. (B) Top view showing the head of the same larvae. Features marked are the eye, the ear and the olfactory bulb (OB; RFP-positive). Boxed area is shown in (C). (C) Confocal image showing the REN and CEN in the hindbrain. Arrows indicate projections to the ALL ganglion and the PLL ganglion. (D) Schematic overview to indicate sites of ALL (S01-SO3; above the eye) and PLL (P1, above the yolk) back-fills. (E) Side view of a PLL back-fill. Back-fills were performed posterior of the PLL ganglion. Note the dendritic process from the ganglion to the brain (above the ear). (F) Overlay of back-filled inhibitory efferent cells in the CEN with the cells labeled by Tg(dmrt3a:GAL4;UAS:GCaMP5g). (F’) Back-fill of ROLE and RELL cells located in the CEN. (F”) Location of overlap by back-filled cells are indicated by white dotted circles in a Nacre Tg(dmrt3a:GAL4;UAS:GCaMP5g) larva. OB, olfactory bulb; ALL, anterior lateral line; PLL, posterior lateral line; REN, the rostral efferent nucleus; CEN, the caudal efferent nucleus nucleus; ROLE, the rhombencephalic octavolateral efferent neuron; RELL, the rhombencephalic efferent neuron to the lateral line.
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