ZFIN ID: ZDB-FIG-210707-69
Ghilardi et al., 2021 - Inner Ear and Muscle Developmental Defects in Smpx-Deficient Zebrafish Embryos. International Journal of Molecular Sciences   22(12) Full text @ Int. J. Mol. Sci.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Antibody:
Fish:
Knockdown Reagents:
Anatomical Term:
Stage: Long-pec
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage Range: Long-pec to Day 4

Figure 2 smpx knockdown disturbs the proper fiber arrangement during development. (A–D) side views, anterior left, dorsal top. Paraffin sections of Control-MO (A,C) and smpx-MO (B,D) injected embryos at 48 hpf (A,B) and 4 dpf (C,D). The red asterisks indicate alterations in the muscle array. (E–H) side views, anterior left, dorsal top. Confocal Z-stacks taken from whole-mount embryos (48 hpf) and larvae (72 hpf) labelled with (E,F) myosin heavy chain (MHC) antibody for slow fibers and (G,H) phalloidin (act) for actin filaments of the fast fibers. The red (F) and white (H) asterisks indicate alterations in the normal muscle array (controls in (E,G), respectively). (I,J) representative lateral view of an embryo injected with the construct encoding the Smpx:GFP chimera (see Figure S1A for construct details); muscle fibers (arrowhead) and epithelial cells (arrow) are both uniformly painted with no accumulation of Smpx:GFP in the nuclei. Inset: magnification of a slow fiber with the nucleus (asterisk) labelled with DAPI (blue). Anterior left, dorsal top. Scale bars = 25 μm in (A–D); 20 μm in (E,F); 20 μm in (G,H); 100 μm in (I,J).

Gene Expression Details No data available
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab-A4.1025 IHC AB control Long-pec slow muscle cell
IHC AB + MO1-smpx control Long-pec slow muscle cell
IHC AB + MO2-smpx control Long-pec slow muscle cell
Phenotype Details
Fish Conditions Stage Phenotype
AB + MO1-smpx control Long-pec fast muscle cell disorganized, abnormal
Long-pec slow muscle cell disorganized, abnormal
Protruding-mouth fast muscle cell disorganized, abnormal
Day 4 fast muscle cell disorganized, abnormal
Day 4 slow muscle cell disorganized, abnormal
AB + MO2-smpx control Long-pec fast muscle cell disorganized, abnormal
Long-pec slow muscle cell disorganized, abnormal
Protruding-mouth fast muscle cell disorganized, abnormal
Day 4 fast muscle cell disorganized, abnormal
Day 4 slow muscle cell disorganized, abnormal
Acknowledgments:
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Int. J. Mol. Sci.