FIGURE 1

Injury-induced changes in level of polyadenylated RNAs. (A) Similarities between transcriptome of RNASeq samples were assessed by hierarchical clustering on the Euclidean distances computed between samples. The RNASeq samples were consistently grouped in their condition, control and injured. (B) RNAs were quantified for all annotated genes in the zebrafish reference genome GRCz11 (“Tested,” n = 32,520), genes expressed in the adult zebrafish telencephalon (“Expressed,” n = 17,301) or genes differentially expressed upon injury (“Differentially expressed”). The blue color depicts increased levels of transcript after injury (n = 1,946) and the yellow color decreased levels of transcript after injury (n = 3,043). (C) The sensitivity of the RNASeq analysis was further evaluated with significant changes in level of RNAs of relevant markers for regenerative neurogenesis. These markers were, selected based on literature, were grouped according to respective biological functions relative to the repair of damaged adult zebrafish telencephalon (color code). *adjp = 0.05, **adjp < 10^{– 02}, ***adjp < 10^{– 04}.