ZFIN Figure: Gunawan et al., 2020, Fig. 3

ZFIN ID: ZDB-FIG-210511-17

ZFIN ID: ZDB-FIG-210511-17

Gunawan et al., 2020 - Nfatc1 Promotes Interstitial Cell Formation During Cardiac Valve Development in Zebrafish. Circulation research 126(8):968-984 Full text @ Circ. Res.

ADDITIONAL FIGURES

EXPRESSION / LABELING:

Genes:	GFP mCherry RFP
Fish:	is4Tg bns321Tg; nkuasrfp1aTg; ubs3Tg cz1701Tg; s898Tg nfatc1^{bns276/bns276}; bns321Tg; nkuasrfp1aTg; ubs3Tg nfatc1^{bns276/bns276}; cz1701Tg; s898Tg nfatc1^{bns276/bns276}; is4Tg
Anatomical Terms:	heart valve heart valve endothelial cell heart valve interstitial cell
Stage Range:	Protruding-mouth to Day 5

PHENOTYPE:

Fish:	nfatc1^{bns276/bns276} (AB) nfatc1^{bns276/bns276}; bns321Tg; nkuasrfp1aTg; ubs3Tg nfatc1^{bns276/bns276}; is4Tg
Observed In:	heart valve interstitial cell heart valve interstitial cell cell proliferation involved in heart valve development pericardium whole organism
Stage Range:	Protruding-mouth to Day 4

Fig. 3 ZFIN is incorporating published figure images and captions as part of an ongoing project. Figures from some publications have not yet been curated, or are not available for display because of copyright restrictions.

Gene Expression Details

Gene	Fish	Conditions	Stage	Anatomy	Assay
GFP	bns321Tg; nkuasrfp1aTg; ubs3Tg	standard conditions	Day 4	heart valve interstitial cell	IFL
	nfatc1^{bns276/bns276}; bns321Tg; nkuasrfp1aTg; ubs3Tg	standard conditions	Day 4	heart valve interstitial cell	IFL
mCherry	cz1701Tg; s898Tg	standard conditions	Day 5	heart valve	IFL
	is4Tg	standard conditions	Protruding-mouth	heart valve endothelial cell	IFL
	nfatc1^{bns276/bns276}; cz1701Tg; s898Tg	standard conditions	Day 5	heart valve	IFL
	nfatc1^{bns276/bns276}; is4Tg	standard conditions	Protruding-mouth	heart valve endothelial cell	IFL
RFP	bns321Tg; nkuasrfp1aTg; ubs3Tg	standard conditions	Day 4	heart valve endothelial cell	IFL
	nfatc1^{bns276/bns276}; bns321Tg; nkuasrfp1aTg; ubs3Tg	standard conditions	Day 4	heart valve endothelial cell	IFL

Antibody Labeling Details No data available

Phenotype Details

Fish	Conditions	Stage	Phenotype
nfatc1^{bns276/bns276} (AB)	standard conditions	Protruding-mouth	whole organism morphology, normal
		Day 4	pericardium edematous, abnormal
nfatc1^{bns276/bns276}; bns321Tg; nkuasrfp1aTg; ubs3Tg	standard conditions	Day 4	heart valve interstitial cell GFP expression decreased distribution, abnormal
nfatc1^{bns276/bns276}; is4Tg	standard conditions	Protruding-mouth	heart valve interstitial cell cell proliferation involved in heart valve development decreased occurrence, abnormal

Full text @ Circ. Res.