FIGURE

Fig. 5

ID

ZDB-FIG-210422-47

Publication

Xie et al., 2021 - Altered Visual Function in a Larval Zebrafish Knockout of Neurodevelopmental Risk Gene pdzk1

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Fig. 5

Transgenic labeling of retinal layers and quantification of retinal morphology for wild-type and pdzk1-KO larvae. (a–b″) Inhibitory neurons, including horizontal and amacrine cells, and their projections into the outer and inner plexiform layers were labeled by transgenic construct Tg(ptf1a:GFP) in green, revealing distinct retinal layers in wild-type and pdzk1-KO retinas. Nuclei stained with DAPI are shown in gray. Scale bar: 50 µm. Normalized (c) IPL thickness, (d) retinal size, (e) retinal thickness, (f) retinal length, and (g) ptf1a positive (ptf1a+) neuron number were quantified. Points are data from individual retinas. White lines represent medians, and dark bands indicate interquartile ranges. For all analyses, unpaired t-tests were performed with 12 and 13 retinas for wild-type and pdzk1-KO groups, respectively. *P < 0.05. INL, inner nuclear layer; IPL, inner plexiform layer; GCL, ganglion cell layer; ON, optic nerve.

Expression Data

Gene:	EGFP
Fish:	jh1Tg pdzk1^uom2/uom2; jh1Tg
Anatomical Terms:	amacrine cell horizontal cell
Stage:	Days 7-13

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	pdzk1^uom2/uom2; jh1Tg
Observed In:	retina retina amacrine cell retinal inner plexiform layer
Stage:	Days 7-13

Phenotype Detail

Acknowledgments

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