FIGURE

Figure 3.

ID

ZDB-FIG-210319-69

Publication

Choi et al., 2021 - Rutin inhibits DRP1-mediated mitochondrial fission and prevents ethanol-induced hepatotoxicity in HepG2 cells and zebrafish

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Figure 3.

The effect of rutin on mitochondrial dynamics in zebrafish larvae. (a) Representative confocal live images of the mitochondria (green fluorescent) with effect of rutin and ethanol on zebrafish larvae muscle. TG(mito:EGFP) zebrafish larvae (4 dpf) were incubated with or without 1 μM rutin under 2% ethanol for 24 h and visualized using a LSM 700 confocal laser microscope (X40). Bar = 10 μm. Quantitative analysis of mitochondrial length in larval muscle cells is presented. Data shown are the means ± SEM of measurements taken from 50 individual cells from three independents experiments. (b) RT-PCR results of mitochondrial fusion and fission related genes affected by rutin following ethanol exposure. (c) Quantitative analysis of relative intensity of drp1, mfn2, and opa1 normalized by gapdh compared to non-treated cells. Data shown are the means ± SEM of measurements taken from three independent experiments. *p < 0.05 and ***p < 0.001.

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Antibody Labeling

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Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Animal Cells Syst (Seoul)