Mitochondrially localized mEos persists for days in neurons. A, Schematic of the photoconversion and tracking strategy. At 4 dpf, pLL axon terminal mitochondria in the NM3 sensory organ (mid-trunk) were photoconverted. At 24, 48, and 72 hpc, the pLLg, proximal axon, and distal axon were imaged. B, C, 24 hpc, mitochondrially localized, converted mEos that originated from the axon terminal is now redistributed to the neuronal cell bodies (magenta or white). We expect two to four neurons have axon terminals in this region at 4 dpf. D–F, 48 hpc, mitochondria converted in NM3 axon terminals are present in the associated cell bodies, proximal, and distal axons of the neuron. G–I, Similarly, 72 hpc mitochondria labeled in the NM3 axon terminals are present throughout the neurons from the cell bodies to the distal axon. J, Quantification of the change in red (converted) fluorescence intensity shows that mitochondrially localized mEos persists in the neuron through 72 hpc (n = 12 larvae each; ANOVA; Tukey's HSD post hoc contrasts). pLLg: 24 hpc: 2.10 ± 0.18; 48 hpc: 1.81 ± 0.38; 72 hpc: 2.69 ± 0.38. Proximal axon: 24 hpc: 1.37 ± 0.16; 48 hpc: 1.75 ± 0.26; 72 hpc: 1.91 ± 0.29; distal axon: 24 hpc: 1.21 ± 0.11; 48 hpc: 1.20 ± 0.09; 72 hpc: 1.08 ± 0.01. Scale bars: 10 µm. All data are mean ± SEM.
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