FIGURE

Fig. 7

ID
ZDB-FIG-210202-15
Publication
Galang et al., 2020 - ATAC-seq Reveals an Isl1 Enhancer that Regulates Sinoatrial Node Development and Function
Other Figures
All Figure Page
Back to All Figure Page
Fig. 7

Figure 7. Evolutionary conservation and upstream regulation of Isl1 sinoatrial node (SAN) enhancer function.A, View of Isl1 locus SAN enhancer (ISE) with alignment to human, mouse, and opossum genomes (above, areas in red indicate >70% conservation, scale from 50% to 100%) and previously published embryonic heart chromatin immunoprecipitation and sequencing (ChIP-seq) data for Gata4 (GATA-binding protein 4), Tbx5 (T-box transcription factor 5), and Tead1 (TEA domain transcription factor 1). B, Maximum intensity projections of confocal z-stacks of 48 hours postfertilization (hpf) Tg(myl7:mCherry-NTR) zebrafish hearts after injection of ISE:green fluorescent protein (GFP) reporter construct (top), or a 1.0-kb fragment (Fr1, lower). C, Enhancer-reporter analysis of founders from injections of ISE and a 1 kb fragment (Fr1 [fragment 1]) in 48 hpf zebrafish hearts with the indicated patterns of reporter activity. Numbers in parentheses indicate the embryos observed to have the indicated expression pattern/total embryos injected that survived to 48 hpf. a indicates atrium; ATAC-seq, assay for transposase-accessible chromatin; avc, av canal; F0, filial generation 0; PC, pacemaker cell; RACM, right atrial cardiomyocyte; san, sinoatrial node; sv, sinus venosus; and v, ventricle.

Expression Data

Expression Detail
Antibody Labeling
Phenotype Data

Phenotype Detail
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Circ. Res.