Figure 2—figure supplement 1.

Frequency at which each nucleotide around the targeted site was deleted.

Nucleotide positions are given in relation to the protospacer adjacent motif (PAM), whose position is set at 0 (0 = PAM nucleotide adjacent to the gRNA binding site). The nucleotide 4 bp before the PAM (−4) was the most frequently deleted (67% of all deletions removed it). n = 4016 unique deletions.

Expression Data

Expression Detail
Antibody Labeling
Phenotype Data

Phenotype Detail
Acknowledgments
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