FIGURE

Figure 1.

ID

ZDB-FIG-201208-45

Publication

Farrugia et al., 2020 - Porphyromonas gingivalis Outer Membrane Vesicles Increase Vascular Permeability

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Figure 1.

Presence and activity of wild-type and ΔK/R-ab Porphyromonas gingivalis gingipains on whole bacteria and outer membrane vesicles (OMVs). (A) Immunopositive bands of 45 kDa were observed in the W83 whole-cell and OMV samples but not in the gingipain-null ΔK/R-ab equivalents when protein extracts were analyzed by immunoblotting using the Rb7 antigingipain antiserum. (B) Cryo–electron microscopy (EM) micrographs showing mAb 1B5 immunogold-labeled W83 bacteria and OMV. The gingipain expression is mainly located to the cell wall in both W83 whole cells and OMVs (black arrows) but is absent in ΔK/R-ab equivalents (scale bar: whole bacteria = 100 nm; OMV = 50 nm). (C–F) Gingipain fluorometric enzyme activity assays showing the higher levels of activity of arginine-specific (Arg, C, E) and lysine-specific (Lys, D, F) protease in W83 whole cells and OMVs compared to ΔK/R-ab mutant equivalents. In C–F, data are mean ± SD of 5 independent experiments with each individual experiment performed in triplicate. Statistical significance was determined by 1-way analysis of variance, ***P < 0.001.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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