FIGURE

Fig 5

ID

ZDB-FIG-200711-27

Publication

Takamiya et al., 2020 - Pax6 organizes the anterior eye segment by guiding two distinct neural crest waves

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Fig 5

Endothelial cells emerge in a concentric ring pattern in the nascent cornea.

(A-C”) The Tg(corneaEndo:GFP) line shows the first corneal endothelium cells (green) as a patch of cells over the lens (arrows in A, 60–72 hpf) that later spread across the entire inner surface of the cornea (B: 96 hpf; C:120 hpf). (C’-C”‘) Transverse (C’) and horizontal (C”‘) optical sections along the stippled lines in C, merged onto pictures from reflection imaging (grey channel) which highlight the corneal epithelium (Ep; note: the strong lateral signals are iridophores). (C”) Magnified view of the central cornea region indicated by a rectangle in C’. En: corneal endothelium; Ln: lens; Ir: iris; Scale bars: 50 μm (A-C, C’ and C”‘); 10 μm (C”). (D) The AS development is classified into five phases. Phase 1: 2°NC cell cluster formation (18–22 hpf); Phase 2: 2°NC cluster dissolves and migrate into the eye (22–28 hpf); Phase 3: NC cells differentiate into ocular mesenchymal cells (28–60 hpf); Phase 4: First corneal endothelial cells emerge over the lens (60–72 hpf); Phase 5: Corneal endothelium fully covers the eye (>72 hpf).

Expression Data

Gene:	GFP
Fish:	ml4Tg
Anatomical Term:	corneal endothelial cell
Stage Range:	Pec-fin to Day 5

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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