FIGURE

Fig. s7

ID

ZDB-FIG-200618-12

Publication

Xie et al., 2019 - Schwann cell precursors contribute to skeletal formation during embryonic development in mice and zebrafish

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Fig. s7

(A-C) In Plp1CreERT2;R26RYFP/+ embryos traced from E12.5 to E17.5 SCPs descendants were detected positive for osterix (OSX) marker for osteo-progenitors in the mandible (A), ribs (B) and scapula (C). Traced cells and OSX+ cells were visualized with an anti-GFP antibody (green) and anti-Osx antibody (red), respectively. The white arrowheads point to the double-positive cells. (D-E) The Plp1CreERT2;R26RYFP/+ embryos traced from E11.5 to E17.5 were stained with an anti-GFP antibody (green) and antibody against a pre-osteoblast marker RUNX2. (F-I) Domains enriched in osteocytes in the mandible (F, H) and scapula (G, I) in Plp1CreERT2;R26RYFP/+ embryos traced from E11.5 to E17.5 were visualized with antibodies against E11/gp38 osteocyte marker (F-G) or dentin matrix protein (DMP1) (H-I). Panels A""-E"" and F"'-I"' depict the same tissue sections as panels A-I, but stained with von Kossa & Alcian blue following completion of confocal scans. The white dashed lines outline the mineralized portion of the bone as indicated by the von Kossa & Alcian blue staining.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Proc. Natl. Acad. Sci. USA