ZFIN ID: ZDB-FIG-200326-44
Jurynec et al., 2019 - The Paf1 Complex and P-TEFb have reciprocal and antagonist roles in maintaining multipotent neural crest progenitors. Development (Cambridge, England)   146(24) Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage Range: Prim-5 to Prim-25
PHENOTYPE:
Fish:
Observed In:
Stage Range: Prim-5 to Prim-25

Fig. 1 alnz24 is a null mutation that regulates NC formation and encodes RNA polymerase II-associated factor 1 (Paf1). (A-E) 36 hpf wild-type and aln mutant embryos. Wild-type embryos (A) have pigmented melanophores lacking in embryos harboring combinations of aln mutant alleles: (B) homozygous for the alnz12 deletion mutation, (C) transheterozygous for the alnz12 and c4 deletions, (D) transheterozygous for the alnz12 deletion and alnz24 ENU-induced mutations, and (E) homozygous for the alnz24 mutation. (F,G) crestin expression detected by whole-mount in situ hybridization in 24 hpf wild-type sibling (F) and alnz24 mutant (G) embryos, indicating a complete absence of migrating trunk NC in alnz24. Arrowheads indicate premigratory and migrating trunk NC. A-G are lateral views with anterior to the left. (H) Paf1 protein is not detected by immunoblot analysis in 36 hpf alnz12 or alnz24 mutant embryos, but is readily detected in wild-type control siblings. Immunoblot detection of pMAPK serves as a protein-loading control.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
crestin WT standard conditions Prim-5 neural crest cell ISH
paf1z24/z24 standard conditions Prim-5 Not Detected neural crest cell ISH
paf1 WT standard conditions Prim-25 whole organism WB
paf1z12Tg/z12Tg standard conditions Prim-25 Not Detected whole organism WB
paf1z24/z24 standard conditions Prim-25 Not Detected whole organism WB
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
paf1z12Tg/z12Tg standard conditions Prim-25 melanocyte unpigmented, abnormal
Prim-25 whole organism paf1 expression absent, abnormal
paf1z12Tg; Df(Chr15:chd)c4 standard conditions Prim-25 melanocyte unpigmented, abnormal
paf1z24/+; paf1z12Tg standard conditions Prim-25 melanocyte unpigmented, abnormal
paf1z24/z24 standard conditions Prim-5 neural crest cell crestin expression absent, abnormal
Prim-25 whole organism paf1 expression absent, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development