ZFIN ID: ZDB-FIG-200313-7
Karjosukarso et al., 2020 - Modeling ZNF408-Associated FEVR in Zebrafish Results in Abnormal Retinal Vasculature. Investigative ophthalmology & visual science   61:39 Full text @ Invest. Ophthalmol. Vis. Sci.
ADDITIONAL FIGURES
PHENOTYPE:
Fish:
Observed In:
Stage Range: Day 5 to Adult

Fig. 4

Progressive vascular pathology in znf408-mutant zebrafish larvae and adults. (a) Confocal micrographs at 5, 90, or 180 days postfertilization (dpf) of the hyaloid (5 dpf, top row) or retinal vessels (90 dpf, second row and 180 dpf, third row) in znf408+/+znf408rmc105/+znf408rmc105/rmc105znf408rmc103/rmc103, and znf408rmc104/rmc104 zebrafish crossed onto the Tg(flk1:GFP) background (vessels shown in green) following injection with rhodamine-conjugated dextran (plasma shown in red). (b) Quantification of the number of hyaloid vessels at 5 dpf from the groups shown in (a). n = 10, ANOVA: P < 0.001, Dunnett's post hoc: ***P < 0.001. (c) Quantification of the number of sprouts in the retinal vessels at 90 dpf from the groups shown in (a). n = 10, ANOVA: P < 0.001, Dunnett's post hoc: ***P < 0.001. (d) Quantification of the leaked dextran as a percentage of total dextran in the retinal vasculature at 180 dpf from the groups shown in (a). n = 10, ANOVA: P < 0.001, Dunnett's post hoc: ***P < 0.001.

Gene Expression Details No data available
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
znf408rmc103/rmc103; la116Tg(TL) standard conditions Day 5 hyaloid vessel decreased amount, abnormal
Adult retina blood vessel has extra parts of type angiogenic sprout, abnormal
Adult retina blood vessel hemorrhagic, abnormal
znf408rmc104/rmc104; la116Tg(TL) standard conditions Day 5 hyaloid vessel decreased amount, abnormal
Adult retina blood vessel has extra parts of type angiogenic sprout, abnormal
Adult retina blood vessel hemorrhagic, abnormal
znf408rmc105/+; la116Tg(TL) standard conditions Day 5 hyaloid vessel decreased amount, abnormal
Adult retina blood vessel morphology, normal
znf408rmc105/rmc105; la116Tg(TL) standard conditions Day 5 hyaloid vessel decreased amount, abnormal
Adult retina blood vessel has extra parts of type angiogenic sprout, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Investigative ophthalmology & visual science for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Invest. Ophthalmol. Vis. Sci.