FIGURE

Figure 7

ID

ZDB-FIG-200302-52

Publication

Otero-Asman et al., 2020 - The extracytoplasmic function sigma factor σ^VreI is active during infection and contributes to phosphate starvation-induced virulence of Pseudomonas aeruginosa

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Figure 7

pdtA mRNA levels upon P. aeruginosa interaction with A549 cells. The P. aeruginosa PAO1 wild-type strain was grown in high Pi medium and inoculated in A549-containing and A549-free cultures. At 3 hpi, total RNA was extracted and pdtA mRNA levels determined by qRT-PCR. Data plotted are the result of eight biologically independent replicates, each bar representing means ± SD of the three technical replicates performed on each biological replicate. The cycle threshold (ct) average and the standard deviation (SD) of each condition is indicated. The 2^−ΔΔCT method was used to determine the fold-change range in pdtA expression in A549-containing versus A549-free cultures. The fold-change range taking into account the SD is shown between brackets.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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