FIGURE

FIGURE 6

ID

ZDB-FIG-200112-16

Publication

Zhuang et al., 2019 - Slc7a14 Is Indispensable in Zebrafish Retinas

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FIGURE 6

Slc7a14 mRNA compensation rescued the phenotypes in slc7a14-deficient morphants. (A) Magnified vertical and lateral views of larval eyeballs. Larvae injected with slc7a14 MO and full-length slc7a14 mRNA showed normal-sized eyeballs at 3 dpf. Scale bar = 100 μm. (B,C) Statistical results for the axial length and ocular area. N = 30 in each group. (D,E) VMR testing showed dramatic recovery of both the ON and OFF responses in slc7a14-deficient zebrafish compensated with mRNA compared to slc7a14-deficient zebrafish without compensation. N = 12 in each group. Rescue experiments were repeated three times. Bar plots are shown as the mean ± s.e.m. Data was analyzed using One-way ANOVA followed by Tukey’s post hoc test, ^∗∗∗P < 0.001 significantly different from slc7a14 6.0 ng group.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + MO1-slc7a14a
Knockdown Reagent:	MO1-slc7a14a
Observed In:	eye optokinetic behavior
Stage:	Protruding-mouth

Phenotype Detail

Acknowledgments

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