FIGURE

Figure 4

ID

ZDB-FIG-200101-11

Publication

Zhang et al., 2019 - Parallel Reaction Monitoring reveals structure-specific ceramide alterations in the zebrafish

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Figure 4

A zebrafish model of Farber disease. (a) Schematic of asah1a/b^−/− zebrafish generation and propagation. (b) DNA fragment analysis demonstrating presence of altered alleles. The 30-bp difference in the asah1b spectra (rather than the noted 20-bp deletion) is due to presence of different polymorphisms across the introns of the WT and KO alleles (Fig. S2b, see Fig. S3 for sequencing of full cDNA). (c) asah1a/b^−/− (DKO) and asah1b^−/− (SKO) zebrafish at 3.5 months. (d) Length of DKO and SKO zebrafish at 3.5–4 months. Zebrafish length measurements: Student t-test, SEM, combined data from n = 13 SKO and n = 12 DKO fish, fish within each data set (n = 4–5 SKO, n = 4 DKO) are age-matched. ***p < 0.001.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	asah1b^f25/f25 asah1a^f24/f24; asah1b^f25/f25
Observed In:	whole organism
Stage:	Adult

Phenotype Detail

Acknowledgments

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