ZFIN ID: ZDB-FIG-191230-559
Kalasekar et al., 2019 - Heterogeneous beta-catenin activation is sufficient to cause hepatocellular carcinoma in zebrafish. Biology Open   8(10) Full text @ Biol. Open
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Term:
Stage: Day 5
PHENOTYPE:
Fish:
Observed In:
Stage: Day 5

Fig. 1.

CreLox system enables switching on activated β-catenin. (A) Schematics of DNA constructs used in this study. Grey boxes indicate promoters; red arrowheads indicate loxP sites. ABC, Activated β-catenin. (B,C) Switching in Tg(fabp10a:Cre) (HepCre),Tg(fabp10a:flox-pt-β-cat) (FloxABC) and Tg(fabp10a:Cre); Tg(fabp10a:flox-pt-β-cat) (HepCre+FloxABC) larvae imaged at 5 dpf for BFP expression, detected by immunofluorescence using an anti-GFP antibody. Successful switching is indicated by loss of BFP expression. (B) Representative images. Livers are outlined. Scale bars: 50 µm. (C) Scatter plot with bar graph quantifying switching in terms of mean intensity of GFP fluorescence, ±standard deviation (s.d.). P-values derived using Sidak's multiple comparisons test following ordinary one-way ANOVA. N values are shown above x axis. Experiment was performed three times, with similar results each time, and representative results are shown. (D,E) Wnt reporter activity in Tg(fabp10a:Cre) (HepCre),Tg(fabp10a:flox-pt-β-cat) (FloxABC) and Tg(fabp10a:Cre); Tg(fabp10a:flox-pt-β-cat) (HepCre+FloxABC) siblings imaged at 5 dpf alongside Tg(fabp10a:pt-β-cat) (HepABC) and non-transgenic siblings (NonTg). All zebrafish contain 7xTCF-Xla.Siam:mCherry transgene for visualization of Wnt reporter activity by immunofluorescence. (D) Representative images of Wnt reporter activity (7xTCF-Xla.Siam:mCherry). Livers are outlined. Scale bars: 50 µm. (E) Stacked bar graphs showing percent of zebrafish with absent (A), low (L, involving <10% of hepatocytes) or high (H, involving >10% of hepatocytes) Wnt reporter activity. P-values derived using Fisher's exact test. N values are shown above x axis. Three experiments were pooled.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
BFP s750Tg ; s752Tg standard conditions Day 5 liver IHC
s752Tg standard conditions Day 5 liver IHC
mCherry ia5Tg ; s704Tg standard conditions Day 5 liver IHC
s750Tg ; s752Tg ; ia5Tg standard conditions Day 5 liver IHC
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
s750Tg ; s752Tg standard conditions Day 5 liver BFP expression decreased amount, abnormal
Constructs: Tg(-3.5ubb:LOXP-EGFP-LOXP-mCherry) , tg(fabp10a:loxp-bfp-loxp-xla.ctnnb1,cryaa:venus) , tg(fabp10a:cre-ert2,cryaa:venus)
Acknowledgments:
ZFIN wishes to thank the journal Biology Open for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Biol. Open