Figure 4—figure supplement 2.

Validation of <italic>Tg(igfbp5a:GCaMP7a)</italic> fish.

(A–B) Embryos injected with BAC(igfbp5a:GCaMP7a) DNA were raised in E3 embryo solution and imaged at 3 dpf before and after the addition of the indicated chemicals (Ionomycin: 5 µM, CaCl2: 10 mM, EGTA: 10 mM).

Expression Data

Expression Detail
Antibody Labeling
Phenotype Data

Phenotype Detail
Acknowledgments
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