FIGURE 2

Social preference test. (A) Social preference. WT, reln^+/– and reln^–/– show a significant preference to spend time near a group of unfamiliar fish [1st strangers; p < 0.0001 for all genotypes; two-way ANOVA followed by Sidak’s post hoc, genotype factor: F(2,62) = 1.818, p = 0.17, stranger factor: F(1,62) = 2392, p < 0.0001, interaction genotype × stranger: F(2,62) = 4.418, p = 0.02]. (B) Preference for social novelty. Both WT and reln^+/– spend an equal amount of time near both groups of unfamiliar fish (1st and 2nd strangers; p = 0.99 for WT and p = 0.38 for reln^+/–). reln^–/– do not switch preference to the second group of unfamiliar fish [2nd strangers; p < 0.0001; two-way ANOVA followed by Sidak’s post hoc, genotype factor: F(2,62) = 0.5184, p = 0.60, stranger factor: F(1,62) = 21.49, p < 0.0001, interaction genotype × stranger: F(2,62) = 6.886, p = 0.002]. n = 12 wild-type, n = 12 reln^+/– and n = 10 reln^–/–. ^****p < 0.0001. Mean ± SEM. (C,D) Photographs showing the experimental setup for social preference (C) and preference for social novelty (D).