FIGURE

Fig. S2

ID

ZDB-FIG-191018-15

Publication

Oikonomou et al., 2019 - The Serotonergic Raphe Promote Sleep in Zebrafish and Mice

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Fig. S2

pCPA treatment depletes brain 5-HT and decreases sleep in zebrafish, related to Figure 1.

(A and B) Maximum intensity projections of dissected brains from 5 dpf zebrafish treated with either DMSO vehicle (A) or pCPA (B) and immunostained for 5-HT. Scale bar, 50 μm; a, anterior; p, posterior; Ra, raphe; Hyp, hypothalamus; Pt, pretectal area.

(C-E) Activity (C), waking activity (D), and sleep (E) for zebrafish treated with DMSO vehicle (blue) or 7.5 μM pCPA (red). Line and shading represent mean ± s.e.m. n = number of animals.

(F and G) Boxplots quantifying activity, waking activity, sleep, sleep bout number, sleep bout length during day

(F) and night (G) and sleep latency during night (G). n = number of animals. ns p>0.05, *p<0.05, **p<0.01, ***p< 0.001, ****p<0.0001, Mann-Whitney test

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Neuron