ZFIN ID: ZDB-FIG-190816-31
Fogerty et al., 2019 - Genomic non-redundancy of the mir-183/96/182 cluster and its requirement for hair cell maintenance. Scientific Reports   9:10302 Full text @ Sci. Rep.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Antibodies:
Fish:
Anatomical Terms:
Stage: Day 5
PHENOTYPE:
Fish:
Observed In:
Stage: Day 5

Fig. 4

Survey of retinal anatomy in 5dpf mutant larvae. Transverse sections were collected near the optic nerve, which is visible in some images (asterisks), and stained with markers for different photoreceptor cell types. In all images, the ventral retina is to the left. (a) Peanut agglutinin lectin (PNA) labels the extracellular matrix surrounding cone outer segments. (b) zpr1 labels red/green cone inner segments. Inserts show higher magnification images, with double arrows indicating how inner segments were measured. (c) zpr3 labels rod outer segments, which are immature at this age and typically longer and more numerous in the ventral retina.

 

Gene Expression Details No data available
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
zpr-1 IHC WT standard conditions Day 5 retinal cone cell photoreceptor inner segment
IHC mir182lri61/lri61 standard conditions Day 5 retinal cone cell photoreceptor inner segment
IHC mir182lri81/lri81 standard conditions Day 5 retinal cone cell photoreceptor inner segment
IHC mir183lri70/lri70 standard conditions Day 5 retinal cone cell photoreceptor inner segment
IHC mir96lri60/lri60 standard conditions Day 5 retinal cone cell photoreceptor inner segment
IHC mir96lri69/lri69 standard conditions Day 5 retinal cone cell photoreceptor inner segment
IHC mir96lri78/lri78 standard conditions Day 5 retinal cone cell photoreceptor inner segment
IHC mir96lri79/lri79 standard conditions Day 5 retinal cone cell photoreceptor inner segment
zpr-3 IHC WT standard conditions Day 5 retinal rod cell rod photoreceptor outer segment
IHC mir182lri61/lri61 standard conditions Day 5 retinal rod cell rod photoreceptor outer segment
IHC mir182lri81/lri81 standard conditions Day 5 retinal rod cell rod photoreceptor outer segment
IHC mir183lri70/lri70 standard conditions Day 5 retinal rod cell rod photoreceptor outer segment
IHC mir96lri60/lri60 standard conditions Day 5 retinal rod cell rod photoreceptor outer segment
IHC mir96lri69/lri69 standard conditions Day 5 retinal rod cell rod photoreceptor outer segment
IHC mir96lri78/lri78 standard conditions Day 5 retinal rod cell rod photoreceptor outer segment
IHC mir96lri79/lri79 standard conditions Day 5 retinal cone cell photoreceptor inner segment
IHC Day 5 retinal rod cell rod photoreceptor outer segment
Phenotype Details
Fish Conditions Stage Phenotype
mir182lri61/lri61 standard conditions Day 5 retinal cone cell morphology, normal
Day 5 retinal cone cell development occurrence, normal
Day 5 retinal rod cell morphology, normal
Day 5 retinal rod cell development occurrence, normal
mir182lri81/lri81 standard conditions Day 5 retinal cone cell morphology, normal
Day 5 retinal cone cell development occurrence, normal
Day 5 retinal rod cell morphology, normal
Day 5 retinal rod cell development occurrence, normal
mir183lri70/lri70 standard conditions Day 5 retinal cone cell morphology, normal
Day 5 retinal cone cell development occurrence, normal
Day 5 retinal rod cell morphology, normal
Day 5 retinal rod cell development occurrence, normal
mir96lri60/lri60 standard conditions Day 5 retinal cone cell morphology, normal
Day 5 retinal cone cell development occurrence, normal
Day 5 retinal rod cell morphology, normal
Day 5 retinal rod cell development occurrence, normal
mir96lri69/lri69 standard conditions Day 5 retinal cone cell morphology, normal
Day 5 retinal cone cell development occurrence, normal
Day 5 retinal rod cell morphology, normal
Day 5 retinal rod cell development occurrence, normal
mir96lri78/lri78 standard conditions Day 5 retinal cone cell photoreceptor inner segment decreased length, abnormal
Day 5 retinal rod cell morphology, normal
Day 5 retinal rod cell development occurrence, normal
mir96lri79/lri79 standard conditions Day 5 retinal cone cell morphology, normal
Day 5 retinal cone cell development occurrence, normal
Day 5 retinal rod cell morphology, normal
Day 5 retinal rod cell development occurrence, normal
Acknowledgments:
ZFIN wishes to thank the journal Scientific Reports for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Sci. Rep.