ZFIN ID: ZDB-FIG-190809-8
Song et al., 2019 - HDAC1-mediated repression of the retinoic acid-responsive gene ripply3 promotes second heart field development. PLoS Genetics   15:e1008165 Full text @ PLoS Genet.
Anatomical Terms:
Stage Range: Prim-15 to Prim-25
Observed In:
Stage Range: Prim-15 to Long-pec

Fig. S10

Generation of ripply3 mutants.

(A) Schematic of the ripply3 exons and the gRNA targeting exon of the ripply3 gene. (B) The ripply3 mutant allele used deletes 61bp including the start codon. (C,D) WT sibling and ripply3mutants at 48 hpf. (E,F) Ripply3 transcripts are not detectable in ripply3 mutants at 30 hpf. Arrows indicate posterior pharyngeal region where ripply3 is expressed in WT embryos. Views are dorsal with anterior up. (G) RT-qPCR for ripply3 in ripply3 mutants at 36 hpf indicates the transcripts are essentially undetectable. Primers used do not bind within the deleted region. (H) Quantification of atrial CMs in crgwt+hetripply3wt+hetcrgwt+hetripply3-/-crg-/-ripply3wt+het, and crg-/-ripply3-/- embryos at 48 hpf (n = 37 for crgwt+hetripply3wt+het, n = 10 for crgwt+hetripply3-/-, n = 48 for crg-/-ripply3wt+het, n = 18 for crg-/-ripply3-/-).

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
ripply3 WT standard conditions Prim-15 pharyngeal arch anatomical region ISH
ripply3ci1010/ci1010 standard conditions Prim-15 Not Detected pharyngeal arch anatomical region ISH
Prim-25 whole organism RTPCR
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
ripply3ci1010/ci1010 standard conditions Prim-15 pharyngeal arch anatomical region ripply3 expression absent, abnormal
Prim-25 whole organism ripply3 expression decreased amount, abnormal
Long-pec whole organism morphology, normal
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