FIGURE

Fig. S6

ID

ZDB-FIG-190726-67

Publication

Plate et al., 2019 - S-Sulfocysteine Induces Seizure-Like Behaviors in Zebrafish

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Fig. S6

Analysis of S-Sulfocysteine long term effects on zebrafish larve

(A) Acridine orange (AO) Staining of 3 dpf zebrafish larve previously treated with 2 mM S-Sulfocysteine (SSC) for 10 min, 30 min, 60 min and untreated control (if not stated otherwise: scalebar 500 um). Representative images are shown.The exposure times used are given in ms for each of the data sets. The untreated larve and larve treated with 2mM SSC for 10 min display auto fluorescence of the yolk and AO staining in regions where apoptosis is part of normal development like the olfactory placode (Van Ham et al., 2010). Larve treated for 30 min with 2mM SSC shoe intense AO staining in the brain. After 60 min of SCC treatment cell death is visualized throughout the whole larve specifying the forebrain (fb) midbrain (mb) and hindbrain (hb) (scalebar 250 um). (B) The head diameter of non treated larve and SSC treated larve were determined. The average head diameter of non treated larve was 423 um +/- 3.68 (=100%). The average head diameter of 2 mM SSC treated larve was found to be significantly higher (136.2 +/- 4.34%). Error bars indicate the standard error of the mean. The results are signifigant with p<0.01.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	AB
Condition:	chemical treatment by environment: S-sulfo-L-cysteine
Observed In:	brain apoptotic process
Stage:	Protruding-mouth

Phenotype Detail

Acknowledgments

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