ZFIN ID: ZDB-FIG-190723-1261
Lessieur et al., 2019 - Ciliary genes arl13b, ahi1 and cc2d2a differentially modify expression of visual acuity phenotypes but do not enhance retinal degeneration due to mutation of cep290 in zebrafish. PLoS One   14:e0213960 Full text @ PLoS One
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Antibodies:
Fish:
Anatomical Terms:
Stage: Adult
PHENOTYPE:
Fish:
Observed In:
Stage: Adult

Fig 10

Combined loss of cep290 and cc2d2a does not exacerbate cone degeneration or rhodopsin trafficking defects.

Panels show immunohistochemical analysis of dorsal retinas from wild-type (top), cep290fh297/fh297 (middle), and cep290fh297/fh297;cc2d2a+/- mutants (bottom) at 6 months of age stained with (A) PNA (red) and Zpr-1 (green) to label cone photoreceptor; (B) Zpr-3 to label rhodopsin; or (C) GRK1 to label rhodopsin kinase. ROS, rod outer segments; COS, cone outer segments; ONL, outer nuclear layer; OPL, outer plexiform layer; INL, inner nuclear layer; IPL, inner plexiform layer; RGC, retinal ganglion cells. Scale bar: 50 μm. (D) Quantification of cone outer segment density or (E) rhodopsin mislocalization from the indicated genotypes at 6 months of age. See methods section for details on quantification. Removing one allele of cc2d2a from a cep290fh297/fh297mutant background had no effect on cone degeneration or rhodopsin mislocalization. At least 10 unique fish over at least 2 independent experiments were evaluated. *** P < 0.0005; **** P < 0.0001 as determined by a 1-way ANOVA with a Multiple Comparisons test and Tukey corrections. Data represented as means ± s.e.m.

Gene Expression Details No data available
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab1-grk1 IHC WT standard conditions Adult retinal rod cell rod photoreceptor outer segment
IHC cc2d2aw38/+ ; cep290fh297/fh297 standard conditions Adult retinal rod cell rod photoreceptor outer segment
IHC cep290fh297/fh297 standard conditions Adult retinal rod cell rod photoreceptor outer segment
zpr-1 IHC WT standard conditions Adult retinal cone cell
IHC cc2d2aw38/+ ; cep290fh297/fh297 standard conditions Adult retinal cone cell
IHC cep290fh297/fh297 standard conditions Adult retinal cone cell
zpr-3 IHC WT standard conditions Adult retinal rod cell rod photoreceptor outer segment
IHC cc2d2aw38/+ ; cep290fh297/fh297 standard conditions Adult photoreceptor inner segment layer
IHC Adult retinal rod cell rod photoreceptor outer segment
IHC cep290fh297/fh297 standard conditions Adult photoreceptor inner segment layer
IHC Adult retinal rod cell rod photoreceptor outer segment
Phenotype Details
Fish Conditions Stage Phenotype
cc2d2aw38/+ ; cep290fh297/fh297 standard conditions Adult photoreceptor inner segment layer zpr-3 labeling mislocalised, abnormal
Adult retinal cone cell decreased amount, abnormal
Adult retinal cone cell cone photoreceptor outer segment decreased amount, abnormal
cep290fh297/fh297 standard conditions Adult photoreceptor inner segment layer zpr-3 labeling mislocalised, abnormal
Adult retinal cone cell decreased amount, abnormal
Adult retinal cone cell cone photoreceptor outer segment decreased amount, abnormal
Acknowledgments:
ZFIN wishes to thank the journal PLoS One for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ PLoS One