Fig. 6
Increase of Neurons, Oligodendrocytes, GFAP-Positive Glia, and Radial Glia in crbn-Overexpressing Larvae (A–C) In situ hybridization analysis of uninjected embryos or those overexpressing gfp, crbnΔMid, or crbnWT. (A) Expression of the neural marker elavl3 (huC) in telencephalon at 24 hpf. (B) Expression of the neural and neural precursor marker neurod1 in telencephalon and lateral longitudinal fascicles at 24 hpf. (C) Expression of the oligodendrocyte marker olig2 in diencephalon at 36 hpf. Upper panels show lower-magnification images, and lower panels show higher-magnification images. (D) 49-hpf larvae immunostained with anti-serotonin antibody. (E) Schematic drawing of serotonin-positive cells at this stage. po, pineal organ; vpt, ventral posterior tubercle; rn, raphe nuclei. (F) The areas of expression domains for elavl3, neurod1, olig2, and serotonin, indicated with dashed lines in (A–C) and (E), were measured and normalized to the value of uninjected embryos and are shown as means ± SD (n = 15 per group). (G) Larvae at 56 hpf immunostained with antibody against the astrocyte marker GFAP. The area surrounded by rectangle in the upper panel is shown in lower panels. The fibers of radial glia are indicated by bracket. (H) Relative amounts of gfap mRNA in 11-hpf embryos were measured by quantitative RT-PCR and are shown as means ± SD (n = 3 per group). Scale bar, 100 μm in (D) and the upper panel in (G) and 50 μm in other panels. **p < 0.01, ***p < 0.001. |