FIGURE

Fig. 1

ID
ZDB-FIG-190715-13
Publication
Williams et al., 2018 - Requirement of zebrafish pcdh10a and pcdh10b in melanocyte precursor migration
Other Figures
All Figure Page
Back to All Figure Page
Fig. 1

pcdh10a is expressed in a subset of crestin positive migratory neural crest cells (NCCs). (A-G) Whole-mount in situ hybridization (ISH) of pcdh10a and crestin expression during NCC migration in wildtype zebrafish embryos. (A) pcdh10a is expressed in a similar pattern to the NCC marker crestin (B) Dorsal views at 18 somites. (C,D) Lateral views at 18 somites. (E, F) Lateral views at 24 hpf. (G) Lateral view at 28 hpf. (H, I, K) Confocal micrograph Z-stack projections of double Fluorescent ISH (FISH) at 23hpf and 26 hpf respectively in wildtype zebrafish embryos coexpressing pcdh10a (Red) and crestin (Green). Lateral views with dorsal top, ventral down. White arrows mark examples of colocalization. Scale bars are 50 µm. (J) Area of imaging for H, I, K, L. (K) Representative image of a 12 µm cross-section of the embryo in (I). White arrows point to areas of colocalization, and white arrowheads point to NCCs in the dorsolateral pathway. (L) 12 µm cross-section through the trunk of an embryo stained for Pcdh10 (Red) immunofluorescence, with neural crest cells expressing sox10:EGFP (Green). Scale bar is 20 µm, images are a single 1 µm Z-plane. NT; Neural Tube, n; Notochord. Anterior is to the left in all whole mount images.

Expression Data
Genes:
Fish:
Anatomical Terms:
Stage Range: 14-19 somites to Prim-5

Expression Detail
Antibody Labeling
Phenotype Data

Phenotype Detail
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image.

Reprinted from Developmental Biology, 444 Suppl 1, Williams, J.S., Hsu, J.Y., Rossi, C.C., Artinger, K.B., Requirement of zebrafish pcdh10a and pcdh10b in melanocyte precursor migration, S274-S286, Copyright (2018) with permission from Elsevier. Full text @ Dev. Biol.