ZFIN ID: ZDB-FIG-190708-41
Breuer et al., 2019 - QDPR homologues in Danio rerio regulate melanin synthesis, early gliogenesis, and glutamine homeostasis. PLoS One   14:e0215162 Full text @ PLoS One
Knockdown Reagent:
Observed In:
Stage: Protruding-mouth

Fig. 4

Reduced brain size but increased number of pH3-positive cells upon qdprb1knockdown.

(A) Dorsal views, anterior to the left. At 3 dpf tg(HuC/D:GFP) transgenic zebrafish show a decreased size of the optic tectum and eye upon qdprb1 knockdown, which can be rescued by co-injecting qdprb1 mRNA. The insets show dorsal views of the left eye, which is reduced in size but still layered upon qdprb1 suppression. This phenotype is rescued upon co-injection of qdprb1 mRNA. The overall GFP signal remains unchanged. (B) The brain area of Qdprb1 hypormorphic embryos is reduced by about 15% compared to wildtypes and rescued by co-injection of qdprb1 mRNA. (C) Z-stack overlays of DAPI (pink) and pH3 (green) staining of dorsally imaged retinas reveal an increased number of pH3-positive retinal cells in Qdprb1 hypormorphic embryos (3 dpf), which are not restricted to the CMZ like found in wildtype retinas. Number and distribution of pH3-positive cells is restored by co-injection of qdprb1 mRNA. (D) Statistical analysis shows a significant increase in pH3 positive cells upon qdprb1knockdown (n = 6) in comparison to wildtype zebrafish (n = 7), which could be rescued by addition of qdprb1 mRNA (n = 3). (E) Z-confocal image of DAPI staining (pink) of the retina at 3 dpf shows retinal layers although decreased overall size and broadened CMZ in qdprb1 hypomorphic embryos.

Gene Expression Details No data available
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
knu3Tg + MO1-qdprb.1 standard conditions Protruding-mouth brain decreased size, abnormal
Protruding-mouth eye cell population proliferation increased process quality, abnormal
Protruding-mouth eye decreased size, abnormal
Protruding-mouth optic tectum cell population proliferation increased process quality, abnormal
Protruding-mouth optic tectum decreased size, abnormal
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