ZFIN ID: ZDB-FIG-190611-15
Delgadillo-Silva et al., 2019 - Modelling pancreatic β-cell inflammation in zebrafish identifies the natural product wedelolactone for human islet protection. Disease models & mechanisms   12(1) Full text @ Dis. Model. Mech.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage: Day 4
PHENOTYPE:
Fish:
Observed In:
Stage: Day 4

Fig. 2

 

Time-lapse imaging reveals dynamic interactions between β-cells and macrophages under chronic inflammation. (A) Representative snapshots from time-lapse movies from control and Tg(ins:il1b) larvae. Tg(ins:mCherry) labels the β-cells (red), whereas Tg(mpeg1:GAL4);Tg(UAS-Kaede) labels the macrophages (green). β-cells and macrophages were imaged every 5 min for 10 h starting at 4.5 dpf. The elapsed time in hours (hrs) is indicated. (B) Plot showing the time that individual macrophages spend in the islet region as defined using a region of interest (ROI) (n=5 animals each for il1b− and il1b+). In controls (blue), the macrophages rarely visit the islet. In Tg(ins:il1b) larvae (red), the macrophages show an increase in the time they spend in the islet region. Unpaired two-tailed t-test with Welch's correction, ****P<0.0001, mean±s.d.

 

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
Kaede gl24Tg; s1999tTg; s948Tg control Day 4 macrophage IFL
gl24Tg; s1999tTg; s948Tg; tud205Tg control Day 4 macrophage IFL
mCherry gl24Tg; s1999tTg; s948Tg control Day 4 islet IFL
gl24Tg; s1999tTg; s948Tg; tud205Tg control Day 4 islet IFL
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
gl24Tg; s1999tTg; s948Tg; tud205Tg control Day 4 islet macrophage increased amount, abnormal
Day 4 islet macrophage migration increased occurrence, abnormal
Acknowledgments:
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