FIGURE

Fig. 2

ID

ZDB-FIG-190329-3

Publication

Pouchucq et al., 2018 - γ-Tubulin small complex formation is essential for early zebrafish embryogenesis

Other Figures

All Figure Page

Back to All Figure Page

Fig. 2

γ-tubulin silencing by morpholino-based knockdown causes developmental arrest during gastrulation. A. Bright field images of the representative phenotypes obtained in a tubg1-Mo (20 nM) injection experiment and complete rescue phenotype. Control injected embryos did not show a phenotype. Images were taken at 8 and 24 hourspost-fertilization (hpf). The frequency of each phenotypic class (severe, moderate and wild type) is indicated as a percentage. The standard deviations correspond to three independent biological replicas. B. Phenotypes distribution of 24 hpf embryos injected with tubg1-Mo (20 and 10 nM), control Mo (20 nM) and tubg1-Mo (20 mM) + γ-tubulin polypeptide.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + MO1-tubg1
Knockdown Reagent:	MO1-tubg1
Observed In:	embryo development whole organism
Stage Range:	75%-epiboly to Prim-5

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image.

Reprinted from Mechanisms of Development, 154, Pouchucq, L., Undurraga, C.A., Fuentes, R., Cornejo, M., Allende, M.L., Monasterio, O., γ-Tubulin small complex formation is essential for early zebrafish embryogenesis, 145-152, Copyright (2018) with permission from Elsevier. Full text @ Mech. Dev.