ZFIN ID: ZDB-FIG-190201-29
Sokol et al., 2018 - Loss of the Mia40a oxidoreductase leads to hepato-pancreatic insufficiency in zebrafish. PLoS Genetics   14:e1007743 Full text @ PLoS Genet.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Antibody:
Fish:
Anatomical Terms:
Stage: Day 4
PHENOTYPE:
Fish:
Observed In:
Stage Range: Day 4 to Day 5

Fig. 3

Abnormal mitochondrial structures are found in the skeletal muscles of mia40a mutants.

(A) Embryos obtained from an in-cross of heterozygous mia40a+/- siblings in the Tg(Xla.Eef1a1:mlsEGFP) background were stained with phalloidin to visualize actin filaments of the skeletal muscle at 4 dpf. Abnormal, enlarged mitochondrial structures are found in the skeletal muscles of the mia40a mutants (arrowheads). To better visualize the GFP-positive inclusions, a magnified image is shown on the side. Images are maximum projections. Scale bar, 50 μm. All images are lateral views, anterior to the left. (B) wild-type and mia40a-/- larvae in the Tg(Xla.Eef1a1:mlsEGFP) background were stained with an anti-Tomm20 antibody to mark the outer mitochondrial membrane (OMM). DNA was counterstained with DAPI. Single plane sections are shown. The arrowhead points to a GFP-positive inclusion. Scale bar, 5 μm. All images are lateral views, anterior to the left. (C) wild-type or mia40a-/- siblings in AB background were subjected to transmission electron microscopy (TEM) analysis at 5 dpf. Arrowheads point to well defined mitochondrial membranes in wild-type larvae. Abnormal membranous structures found in the mia40a mutants are magnified (inset). Scale bar, 500 nm. M: Mitochondria.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
EGFP chchd4abns292/+; cms1Tg standard conditions Day 4 skeletal muscle IHC
Day 4 skeletal muscle mitochondrial matrix IHC
chchd4abns292/bns292; cms1Tg standard conditions Day 4 skeletal muscle IHC
Day 4 skeletal muscle mitochondrial matrix IHC
cms1Tg standard conditions Day 4 skeletal muscle IHC
Day 4 skeletal muscle mitochondrial matrix IHC
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab1-tomm20 IHC chchd4abns292/bns292; cms1Tg standard conditions Day 4 skeletal muscle mitochondrial outer membrane
IHC cms1Tg standard conditions Day 4 skeletal muscle mitochondrial outer membrane
Phenotype Details
Fish Conditions Stage Phenotype
chchd4abns292/bns292 (AB) standard conditions Day 5 skeletal muscle mitochondrion has extra parts of type skeletal muscle inclusion body, abnormal
Day 5 skeletal muscle mitochondrion morphology, abnormal
chchd4abns292/bns292; cms1Tg standard conditions Day 4 skeletal muscle EGFP expression spatial pattern, abnormal
Day 4 skeletal muscle mitochondrion morphology, abnormal
Acknowledgments:
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ PLoS Genet.