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ZIRC
ZFIN ID: ZDB-FIG-190201-14
Naylor et al., 2018 - A novel mechanism of gland formation in zebrafish involving transdifferentiation of renal epithelial cells and live cell extrusion. eLIFE   7 Full text @ Elife
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Antibody:
Fish:
Condition:
Knockdown Reagents:
Anatomical Terms:
Stage Range: Prim-5 to Long-pec
PHENOTYPE:
Fish:
Condition:
Knockdown Reagents:
Observed In:
Stage: Long-pec

Fig. 6

Notch inhibition precludes CS gland formation.

(A) Panels show a stage series of fluorescent images of the TP1:VenusPEST transgenic line in the trunk region (arrows mark fluorescence in the region of the CS gland). (B) Left panels show lateral views of the posterior trunk showing stc1 expression after the indicated treatments. Middle panels show transverse sections of the pronephric tubule and position of the CS gland showing Hnf1b staining (±DAPI) after the same treatments. Right panels show lateral views of the trunk in the region of the CS gland (highlighted by unfilled arrow). (C) Histogram showing ‘corrected total nuclear fluorescence’ (CTNF) measurements of nuclei in the positions indicated. (D) Histograms showing the number of embryos with fluorescence in the CS gland in progeny from TP1:VenusPEST in-crosses with the indicated treatments.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
stc1 TU control Long-pec corpuscles of Stannius ISH
TU chemical treatment by environment: compound E Long-pec corpuscles of Stannius ISH
TU + CRISPR1-irx3b + CRISPR2-irx3b control Long-pec corpuscles of Stannius ISH
Long-pec pronephric distal early tubule ISH
TU + CRISPR1-irx3b + CRISPR2-irx3b chemical treatment by environment: compound E Long-pec corpuscles of Stannius ISH
Long-pec pronephric distal early tubule ISH
Venus s940Tg control Long-pec corpuscles of Stannius IFL
s940Tg standard conditions Prim-5 Not Detected corpuscles of Stannius IFL
Prim-5 Not Detected pronephric distal early tubule IFL
Prim-5 Not Detected pronephric distal late tubule IFL
Prim-25 corpuscles of Stannius IFL
Prim-25 Not Detected pronephric distal early tubule IFL
Prim-25 Not Detected pronephric distal late tubule IFL
High-pec corpuscles of Stannius IFL
High-pec Not Detected pronephric distal early tubule IFL
High-pec Not Detected pronephric distal late tubule IFL
Long-pec corpuscles of Stannius IFL
Long-pec Not Detected pronephric distal early tubule IFL
Long-pec Not Detected pronephric distal late tubule IFL
s940Tg chemical treatment by environment: compound E Long-pec Not Detected corpuscles of Stannius IFL
s940Tg + CRISPR1-irx3b + CRISPR2-irx3b control Long-pec corpuscles of Stannius IFL
Long-pec pronephric distal early tubule IFL
s940Tg + CRISPR1-irx3b + CRISPR2-irx3b chemical treatment by environment: compound E Long-pec Not Detected corpuscles of Stannius IFL
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab2-hnf1b IHC TU control Long-pec corpuscles of Stannius cytoplasm
IHC Long-pec pronephric distal early tubule nucleus
IHC TU chemical treatment by environment: compound E Long-pec pronephric distal early tubule nucleus
IHC TU + CRISPR1-irx3b + CRISPR2-irx3b control Long-pec pronephric distal early tubule cytoplasm
IHC TU + CRISPR1-irx3b + CRISPR2-irx3b chemical treatment by environment: compound E Long-pec pronephric distal early tubule nucleus
Phenotype Details
Fish Conditions Stage Phenotype
TU chemical treatment by environment: compound E Long-pec corpuscles of Stannius stc1 expression decreased amount, abnormal
Long-pec corpuscles of Stannius embryonic organ development decreased process quality, abnormal
TU + CRISPR1-irx3b + CRISPR2-irx3b control Long-pec corpuscles of Stannius embryonic organ development decreased process quality, abnormal
Long-pec pronephric distal early tubule stc1 expression increased distribution, abnormal
Long-pec pronephric distal early tubule stc1 expression mislocalised, abnormal
Long-pec pronephric distal early tubule cytoplasm Ab2-hnf1b labeling mislocalised, abnormal
TU + CRISPR1-irx3b + CRISPR2-irx3b chemical treatment by environment: compound E Long-pec corpuscles of Stannius stc1 expression decreased amount, abnormal
Long-pec corpuscles of Stannius embryonic organ development decreased process quality, abnormal
Long-pec pronephric distal early tubule stc1 expression increased distribution, abnormal
s940Tg chemical treatment by environment: compound E Long-pec corpuscles of Stannius Venus expression absent, abnormal
Long-pec corpuscles of Stannius embryonic organ development decreased process quality, abnormal
s940Tg + CRISPR1-irx3b + CRISPR2-irx3b control Long-pec corpuscles of Stannius embryonic organ development decreased process quality, abnormal
Long-pec pronephric distal early tubule Venus expression increased distribution, abnormal
Long-pec pronephric distal early tubule Venus expression mislocalised, abnormal
s940Tg + CRISPR1-irx3b + CRISPR2-irx3b chemical treatment by environment: compound E Long-pec corpuscles of Stannius Venus expression absent, abnormal
Long-pec corpuscles of Stannius embryonic organ development decreased process quality, abnormal
Acknowledgments:
ZFIN wishes to thank the journal eLIFE for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Elife