ZFIN ID: ZDB-FIG-190129-22
Yamaguchi et al., 2018 - Systematic studies of all PIH proteins in zebrafish reveal their distinct roles in axonemal dynein assembly. eLIFE   7 Full text @ Elife
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Antibodies:
Fish:
Anatomical Terms:
Stage: Adult
PHENOTYPE:
Fish:
Observed In:
Stage: Adult

Fig. 4

Immunoblot and immunofluorescence microscopy of axonemal dyneins.

(A) Immunoblot of sperm axonemes. Asterisks, filled circles, and open circles indicate missing, decreased, and shifted bands, respectively. Acetylated tubulin: control. (B) Immunofluorescence microscopy of zebrafish spermatozoa. Dnah8 was localized along the entire length of sperm flagella in WT, pih1d1-/-, pih1d2-/-, ktu-/-. In twister-/-, Dnah8 was lost (white asterisk), while in pih1d2-/-;ktu-/-, Dnah8 was localized only in the proximal half of the flagella (white arrowhead).

Gene Expression DetailsNo data available
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Qualifier Anatomy
Ab1-dnah2 WB WT standard conditions Adult sperm axoneme
WB dnaaf2ja104/ja104(TL) standard conditions Adult sperm axoneme
WB pih1d1ja102/ja102 standard conditions Adult sperm axoneme
WB pih1d2ja103/ja103 standard conditions Adult sperm axoneme
WB pih1d2ja103/ja103 ; dnaaf2ja104/ja104 standard conditions Adult sperm axoneme
WB pih1d3ja105/ja105(TL) standard conditions Adult sperm axoneme
Ab1-dnah5l IHC WT standard conditions Adult sperm sperm flagellum
IHC dnaaf2ja104/ja104(TL) standard conditions Adult sperm sperm flagellum
IHC pih1d1ja102/ja102 standard conditions Adult sperm sperm flagellum
IHC pih1d2ja103/ja103 standard conditions Adult sperm sperm flagellum
IHC pih1d2ja103/ja103 ; dnaaf2ja104/ja104 standard conditions Adult sperm sperm flagellum
IHC pih1d3ja105/ja105(TL) standard conditions Adult Not Detected sperm sperm flagellum
WB WT standard conditions Adult sperm axoneme
WB dnaaf2ja104/ja104(TL) standard conditions Adult sperm axoneme
WB pih1d1ja102/ja102 standard conditions Adult sperm axoneme
WB pih1d2ja103/ja103 standard conditions Adult sperm axoneme
WB pih1d2ja103/ja103 ; dnaaf2ja104/ja104 standard conditions Adult sperm axoneme
WB pih1d3ja105/ja105(TL) standard conditions Adult Not Detected sperm axoneme
Ab1-dnai1 WB WT standard conditions Adult sperm axoneme
WB dnaaf2ja104/ja104(TL) standard conditions Adult sperm axoneme
WB pih1d1ja102/ja102 standard conditions Adult sperm axoneme
WB pih1d2ja103/ja103 standard conditions Adult sperm axoneme
WB pih1d2ja103/ja103 ; dnaaf2ja104/ja104 standard conditions Adult sperm axoneme
WB pih1d3ja105/ja105(TL) standard conditions Adult Not Detected sperm axoneme
Ab1-dnali1 WB WT standard conditions Adult sperm axoneme
WB dnaaf2ja104/ja104(TL) standard conditions Adult sperm axoneme
WB pih1d1ja102/ja102 standard conditions Adult sperm axoneme
WB pih1d2ja103/ja103 standard conditions Adult sperm axoneme
WB pih1d2ja103/ja103 ; dnaaf2ja104/ja104 standard conditions Adult sperm axoneme
WB pih1d3ja105/ja105(TL) standard conditions Adult sperm axoneme
Phenotype Details
Fish Conditions Stage Phenotype
dnaaf2ja104/ja104(TL) standard conditions Adult sperm axoneme Ab1-dnali1 labeling decreased amount, abnormal
pih1d1ja102/ja102 standard conditions Adult sperm axoneme Ab1-dnali1 labeling decreased amount, abnormal
pih1d2ja103/ja103 ; dnaaf2ja104/ja104 standard conditions Adult sperm axoneme Ab1-dnali1 labeling decreased amount, abnormal
pih1d2ja103/ja103 ; dnaaf2ja104/ja104 standard conditions Adult sperm axoneme Ab1-dnah5l labeling decreased amount, abnormal
pih1d2ja103/ja103 ; dnaaf2ja104/ja104 standard conditions Adult sperm axoneme Ab1-dnai1 labeling decreased amount, abnormal
pih1d2ja103/ja103 ; dnaaf2ja104/ja104 standard conditions Adult sperm sperm flagellum Ab1-dnah5l labeling decreased distribution, abnormal
pih1d2ja103/ja103 ; dnaaf2ja104/ja104 standard conditions Adult sperm sperm flagellum Ab1-dnah5l labeling spatial pattern, abnormal
pih1d3ja105/ja105(TL) standard conditions Adult sperm axoneme Ab1-dnai1 labeling absent, abnormal
Adult sperm axoneme Ab1-dnah5l labeling absent, abnormal
Adult sperm axoneme Ab1-dnali1 labeling decreased amount, abnormal
Adult sperm sperm flagellum Ab1-dnah5l labeling absent, abnormal
Acknowledgments:
ZFIN wishes to thank the journal eLIFE for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Elife