ZFIN ID: ZDB-FIG-190107-3
Blanco-Sánchez et al., 2018 - Grxcr1 Promotes Hair Bundle Development by Destabilizing the Physical Interaction between Harmonin and Sans Usher Syndrome Proteins. Cell Reports   25:1281-1291.e4 Full text @ Cell Rep.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Antibodies:
Fish:
Anatomical Terms:
Stage: Day 5

Fig. 5

Grxcr1 Localizes in the Golgi Apparatus and ERGIC of the Hair Cells

(A–C) Confocal sections of the anterior macula. Shown are partial colocalization of Grxcr1 (red) and phalloidin (A), GM130 (B), or KDEL (green, C). Individual hair cells are outlined in white. Blue, red, and yellow arrows show Grxcr1 localization in the cell body, hair bundles, and kinocilia, respectively. The confocal sections were selected based on the localization of the organelles of interest (hair bundles, Golgi, and ER), hence the differences in Grxcr1 localization. Nuclei are outlined in blue; 5 dpf.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
grxcr1a Ab1-grxcr1a WT standard conditions Day 5 hair cell anterior macula cell body IHC
Ab1-grxcr1a Day 5 hair cell anterior macula kinocilium IHC
Ab1-grxcr1a Day 5 hair cell anterior macula stereocilium IHC
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab1-golga2 IHC WT standard conditions Day 5 hair cell anterior macula cis-Golgi network
IHC Day 5 hair cell anterior macula endoplasmic reticulum-Golgi intermediate compartment
Ab3-hspa5 IHC WT standard conditions Day 5 hair cell anterior macula endoplasmic reticulum
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal Cell Reports for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Cell Rep.