ZFIN ID: ZDB-FIG-181207-37
Tessadori et al., 2018 - Effective CRISPR/Cas9-based nucleotide editing in zebrafish to model human genetic cardiovascular disorders. Disease models & mechanisms   11(10) Full text @ Dis. Model. Mech.
ADDITIONAL FIGURES
PHENOTYPE:
Fish:
Observed In:
Stage: Day 5

Fig. S10

abcc9[G989E] embryos show CS-related anomalous heart function and pericardial edema at 5 dpf. (A) Comparison of wild-type and abcc9[G989E] mutants at 5 dpf seen from a left lateral (left) and dorsal view (right). (B-D) Quantification of cardiac function using sequential still frames from high-speed imaging of the embryonic cardiac cycle at 5 dpf. Ventricular volume (B), cardiac output (C) and contractile function (D) were calculated. (E) Quantification of pericardial edema by measuring pericardial area using striking morphological landmarks. In order to correct for possible enlarged ventricular size in mutants, ventricular area was subtracted. (F) Quantification of vascular dilations in a Tg(kdrl:GFP) background. 3D reconstruction of vascular structure in Imaris was used to calculate vessel volume. For all graphs, significance was determined by two-tailed unpaired Student's t test or Mann–Whitney two-tailed U test: * p≤0.05; ** p≤0.01; *** p≤0.001; **** p≤0.0001. The black horizontal bar indicates the mean value for each condition. Sample size, abcc9+/+, n=20; abcc9+/G989E, n=17; abcc9G989E/G989E, n=19 in B-E; abcc9+/+, n=5; abcc9+/G989E, n=10; abcc9G989E/G989E, n=5 in F. Scale bar, 1 mm in A. All embryos analyzed originated from group matings of adult zebrafish.

Gene Expression Details No data available
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
abcc9hu11838/+ standard conditions Day 5 heart process process quality, abnormal
Day 5 pericardium edematous, abnormal
abcc9hu11838/hu11838 standard conditions Day 5 heart process process quality, abnormal
Day 5 pericardium edematous, abnormal
la116Tg ; abcc9hu11859/+ standard conditions Day 5 basal communicating artery increased curvature, abnormal
Day 5 basal communicating artery kinked, abnormal
Day 5 posterior communicating artery increased curvature, abnormal
Day 5 posterior communicating artery kinked, abnormal
la116Tg ; kcnj8hu11872/+ standard conditions Day 5 basal communicating artery increased curvature, abnormal
Day 5 basal communicating artery kinked, abnormal
Day 5 posterior communicating artery increased curvature, abnormal
Day 5 posterior communicating artery kinked, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Disease models & mechanisms for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Dis. Model. Mech.