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ZFIN ID: ZDB-FIG-181207-30
Weger et al., 2018 - Glucocorticoid deficiency causes transcriptional and post-transcriptional reprogramming of glutamine metabolism. EBioMedicine   36:376-389 Full text @ EBioMedicine
Anatomical Term:
Stage: Day 5
Observed In:
Stage: Day 5

Fig. 6

fdx1b−/− mutant larvae exhibit hallmarks of primary adrenal insufficiency and differ from rx3 strong mutant larvae reminiscent to secondary adrenal insufficiency.

(A) Schematic shows the different alterations in the pituitary and interrenal gland axis of fdx1b−/− and rx3 strong mutant larvae. Abbreviations: Pomca, pro-opiomelanocortin; ACTH, adrenocorticotropin; SG, steroidogenesis. (B) Whole-mount in situ hybridization of cyp17a2 to visualize the interrenal gland (arrow) in fdx1b−/− mutant larvae (n = 17) and wild-type siblings (n = 11) at 120 hpf stage. Bar plot shows the relative (rel.) changes in the diameter of the interrenal gland size. (C) Barcode plots for ACTH target genes in fdx1−/− mutant larvae (left) and rx3 strong mutant larvae (right). The two mutant types show opposing directions in the gene set enrichment (p-value = 1.7 E−04) for gene set enrichment analysis. (D) Gene set enrichment analysis of differentially expressed genes showing different regulation between fdx1b−/− and rx3 strong. (E) Barcode plots for metabolic pathways and Nrf2 target genes in fdx1b−/− mutant larvae (left) and rx3 strong mutant larvae (right) in comparison to the corresponding control (wild-type siblings or rx3 weak). (F) Fold changes of differentially expressed genes in steroid hormone biosynthesis between mutant larvae and their corresponding controls. The data for the rx3 mutants is a reanalysis from our previously published data set [74]. Scale bar = 110 μm. *, p < .05, **, p < .01, ***, p < .001.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
cyp17a2 WT standard conditions Day 5 interrenal gland ISH
fdx1buob205/uob205 standard conditions Day 5 interrenal gland ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
fdx1buob205/uob205 standard conditions Day 5 interrenal gland hyperplastic, abnormal
Day 5 interrenal gland cyp17a2 expression increased distribution, abnormal
Day 5 interrenal gland increased size, abnormal
ZFIN wishes to thank the journal EBioMedicine for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ EBioMedicine