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ZFIN ID: ZDB-FIG-181023-8
Grassini et al., 2018 - Nppa and Nppb act redundantly during zebrafish cardiac development to confine AVC marker expression and reduce cardiac jelly volume. Development (Cambridge, England)   145(12) Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage Range: Long-pec to Protruding-mouth

Fig. 3

A transgenic biosensor for the cardiac jelly. (A) Lateral view of the Tg(ubi:ssNcan-EGFP) line: a biosensor for hyaluronic acid – a major constituent of the cardiac jelly. The sensor expresses a secreted fusion protein of the HA-binding domain of Neurocan fused to GFP, driven by the ubi promoter. The biosensor localises to regions of HA, including the cardiac jelly, the otic vesicle and around the vasculature. (B) The Tg(ubi:ssEGFP) control was also generated and shows different EGFP localisation, predominantly to the skeletal muscle. Scale bars: 100 µm. (C,C′) Frontal view images of 48 hpf Tg(ubi:ssNcan-EGFP)/Tg(kdrl:mCherry) hearts anaesthetised and live imaged showing the cardiac jelly (green) and endocardial layer (red). ssNcan-EGFP localises to mucous cells (black arrowhead) and is concentrated at the AVC (asterisks). Regions devoid of ssNcan-EGFP in the cardiac jelly due to protruding endocardial cells can also be observed (white arrowheads). (D,D′) Live images of 48 hpf Tg(ubi:ssEGFP)/Tg(kdrl:mCherry) hearts showing near undetectable amounts of ssEGFP localisation (for full z-stacks of C,D, see Movie 2). (E,E′) Live images of 72 hpf Tg(ubi:ssNcan-EGFP)/Tg(kdrl:mCherry) ventricles showing condensed jelly and sites of trabeculae formation. (F,F′) Live images of 72 hpf Tg(ubi:ssEGFP)/Tg(kdrl:mCherry) hearts showing ssEGFP localisation in the pericardial space. Scale bar: 20 µm.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
EGFP s916Tg ; uq25bhTg standard conditions Long-pec atrioventricular canal cardiac jelly IFL
Long-pec mucus secreting cell IFL
Protruding-mouth atrioventricular canal cardiac jelly IFL
uq25bhTg standard conditions Protruding-mouth axial vasculature extracellular matrix IFL
Protruding-mouth cardiac jelly IFL
Protruding-mouth inner ear extracellular matrix IFL
mCherry s916Tg ; uq25bhTg standard conditions Long-pec endocardium IFL
Protruding-mouth endocardium IFL
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development