FIGURE

Fig. 1

ID

ZDB-FIG-180713-34

Publication

Yan et al., 2018 - Activation of Hepatic Stellate Cells During Liver Carcinogenesis Requires Fibrinogen/Integrin αvβ5 in Zebrafish

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Fig. 1

Characterization of kras^V12-induced hepatocarcinogenesis in zebrafish larvae. 3-dpi kras+ or WT larvae were treated with 20 μg/ml for 4 days. More than 20 larvae were analyzed in each group. (A) Gross morphology of kras+ or WT larvae after 4 days induction (left lateral view). kras^V12 expressing liver is marked by GFP expression and WT liver is outlined. Quantification of 2D liver size was shown in the right panel. (B-E) IF staining of PCNA (B), Caspase 3 (C), Collagen I (D) or Laminin (E) on liver sections of kras+ and WT larvae as indicated. Livers are marked by dash lines. Int indicates intestine. Quantifications of staining signals based on percentages of liver area are shown in the right panels. (F) IF co-staining of GFAP (red) and a-Sma (blue) on liver sections of kras+ and WT larvae as indicated. Quantifications of total HSC density based on Gfap+ cells and ratio of activated HSCs based on a-SMA staining in liver sections are presented on the right. *P < .05. Scale bars: 20 μm.

Expression Data

Antibodies:	Ab1-casp3 Ab1-lama1 Ab2-col1 Ab5-acta2 Ab5-pcna
Fish:	WT gz32Tg
Condition:	chemical treatment by environment: doxycycline
Anatomical Terms:	hematopoietic stem cell liver
Stage:	Days 7-13

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT gz32Tg
Condition:	chemical treatment by environment: doxycycline
Observed In:	hematopoietic stem cell liver
Stage:	Days 7-13

Phenotype Detail

Acknowledgments

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