FIGURE

Fig. 4

ID

ZDB-FIG-180627-10

Publication

Cheng et al., 2018 - The long non-coding RNA uc.4 influences cell differentiation through the TGF-beta signaling pathway

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Fig. 4

The overexpression of uc.4 caused abnormal development of zebrafish. (a) The overexpression of uc.4 increased the death rates of zebrafish in a dose-dependent manner. (b) The death rates of the WT, NC and uc.4 groups (200 pg). (c) The expression of uc.4 was upregulated in the uc.4 overexpression groups. ***P<0.001 versus NC groups, Student’s t-test. (d) The overexpression of uc.4 was determined by in situ hybridization. uc.4 is primarily distributed in the atria of zebrafish. (e) The overexpression of uc.4 leads to heart looping defects and pericardial edema in zebrafish. (f) qRT-PCR determination of the mRNA expression of cardiac-specific markers (Nkx2.5, Vmhc, GATA4, hand2, Mef2c, Cx43). A minor decrease was observed in the expression of these cardiac markers. *P<0.05, **P<0.01 versus NC groups, Student’s t-test. Each experiment was repeated three times. Data are presented as the means±s.d. NC, negative control; qRT-PCR, quantitative real-time polymerase chain reaction.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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