FIGURE

Fig. 7

ID
ZDB-FIG-180622-3
Publication
Hu et al., 2018 - Comparison of Various Nuclear Localization Signal-Fused Cas9 Proteins and Cas9 mRNA for Genome Editing in Zebrafish.
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Fig. 7

Multiplex knockout efficiency of various nuclear localization signal (NLS)-fused Cas9 proteins and N-Cas9-C mRNA. Survival rates of zebrafish embryos at 24 and 48 hr postfertilization (hpf) (A) and T7 Endonuclease I (T7E1) assays of the rates of mutagenesis for each site (B). The phenotypes observed were wild-type (WT) (C), severe mosaic hypopigmentation (D), and null-like (E). (F) Proportions of embryos with each phenotype. Scale bars: 0.2 mm.

Expression Data

Expression Detail
Antibody Labeling
Phenotype Data

Phenotype Detail
Acknowledgments
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