FIGURE

Fig. S9

ID

ZDB-FIG-180620-37

Publication

Novas et al., 2018 - Kinesin 1 regulates cilia length through an interaction with the Bardet-Biedl syndrome related protein CCDC28B

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Fig. S9

ccdc28b CRISPR/Cas9 Fo zebrafish. A) PCR DNA analysis of individual embryos injected with two ccdc28b sgRNAs. In both cases the PCR result is compatible with the gRNAs driving genomic changes in the locus B) CRISPR/Cas9 genome editing of ccdc28b generated embryonic phenotypes similar to those observed in embryos injected with ccdc28b morpholinos. The let cloumn shows low magnification images of the general morphology of embryos injected with the combination of sgRNA and zf-nCas9n mRNA at 48 hpf. Phenotypes resemble those found in embryos injected with morpholinos. Embryos with similar phenotypes were fixed to analyze the aspect of cilia in different organs through immunofluorescence. Actin and cilia axoneme were stained with TMR conjugated phalloidin (magenta) and anti-acetylated tubulin respectively(green). As in embryos injected with morpholinos, embryos injected with sgRNA targeting ccdc28b showed perturbed ciliated tissues.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + CRISPR1-ccdc28b + CRISPR2-ccdc28b
Knockdown Reagents:	CRISPR1-ccdc28b CRISPR2-ccdc28b
Observed In:	eye olfactory pit otic vesicle pigmentation trunk whole organism anterior-posterior axis
Stage:	Long-pec

Phenotype Detail

Acknowledgments

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