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ZFIN ID: ZDB-FIG-180510-38
Arévalo et al., 2018 - Generation and Characterization of Antibodies against Opioid Receptors from Zebrafish. International Journal of Molecular Sciences   19(1) Full text @ Int. J. Mol. Sci.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Antibodies:
Fish:
Knockdown Reagents:
Anatomical Terms:
Stage: Long-pec
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage: Long-pec

Fig. 4

Antibodies against MOR, DOR1 and DOR2 recognize endogenous receptors in zebrafish embryos by immunohistochemistry. (A) Lateral and dorsal views of opioid receptors protein distribution in zebrafish embryos by whole-mount immunohistochemistry at 48 hpf. Embryos were processed as described in the Material and Methods section and subjected to immunohistochemistry with rabbit IgGs (Control), MOR, DOR1 or DOR2 antibodies for panels a, b, c and d, respectively. Magnified trunk (a′, b′, c′ and d′) and head panels (a′′, b′′, c′′ and d′′) are shown. Scale bar: 250 µm. Abbreviations: T: telencephalon; D: diencephalon; Hb: hindbrain; red asterisk: somite. A representative experiment is shown (n = 3); (B) Lateral and dorsal views of opioid receptors protein distribution in zebrafish embryos injected with the corresponding morpholino by whole-mount immunohistochemistry at 48 hpf. Embryos were injected with morpholinos specific to different opioid receptors at the one-to-four-cell stage in the yolk (panels a, b and c). Magnified trunk (a′, b′ and c′) and head panels (a′′, b′′ and c′′) are shown. Scale bar: 250 µm. Note the reduced signal in embryos injected with the corresponding morpholino compared to non-injected embryos (panel (A)). A representative experiment is shown (n = 3). (C) Lateral and dorsal views of opioid receptors mRNA distribution in zebrafish embryos by whole-mount in situ hybridization at 48 hpf. Expression of opioid receptor mRNA using no probe (a) and antisense probes against MOR (b), DOR1 (c) and DOR2 (d) are shown. Magnified trunk (b′, c′ and d′) and head panels (b′′, c′′ and d′′) are shown; red asterisk: somite. Scale bar: 250 µm. A representative experiment is shown (n = 3).

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
oprd1a Ab1-oprd1a AB standard conditions Long-pec brain IHC
Ab1-oprd1a Long-pec central nervous system IHC
Long-pec diencephalon ISH
Long-pec hindbrain ISH
Long-pec telencephalon ISH
Ab1-oprd1a AB + MO1-oprd1a standard conditions Long-pec brain IHC
Ab1-oprd1a Long-pec central nervous system IHC
oprd1b Ab1-oprd1b AB standard conditions Long-pec brain IHC
Ab1-oprd1b Long-pec central nervous system IHC
Long-pec diencephalon ISH
Long-pec hindbrain ISH
Long-pec telencephalon ISH
Ab1-oprd1b AB + MO1-oprd1b standard conditions Long-pec brain IHC
Ab1-oprd1b Long-pec central nervous system IHC
oprm1 Ab1-oprm1 AB standard conditions Long-pec brain IHC
Ab1-oprm1 Long-pec central nervous system IHC
Long-pec diencephalon ISH
Long-pec hindbrain ISH
Long-pec telencephalon ISH
Ab1-oprm1 AB + MO1-oprm1 standard conditions Long-pec brain IHC
Ab1-oprm1 Long-pec central nervous system IHC
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
AB + MO1-oprd1a standard conditions Long-pec brain oprd1a expression decreased amount, abnormal
Long-pec central nervous system oprd1a expression decreased amount, abnormal
AB + MO1-oprd1b standard conditions Long-pec brain oprd1b expression decreased amount, abnormal
Long-pec central nervous system oprd1b expression decreased amount, abnormal
AB + MO1-oprm1 standard conditions Long-pec brain oprm1 expression decreased amount, abnormal
Long-pec central nervous system oprm1 expression decreased amount, abnormal
Acknowledgments:
ZFIN wishes to thank the journal International Journal of Molecular Sciences for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Int. J. Mol. Sci.