Fig. S2

Characterization of THOR Transcript, Coding Potential, and Tissue Expression, Related to Figure 1

(A) Northern blot of endogenous THOR in H1299 cells, and of H1437 cells expressing LacZ control, THOR, and THOR with the addition of siRNA targeting THOR. Blot of gapdh provided as a control.

(B) Bar plot depicting the qPCR expression of the long versus short THOR isoform.

(C) qPCR expression of the long THOR isoform following addition of siRNA.

(D) Northern blot of THOR in zebrafish kidney and testis. Blot of GAPDH provided as a control.

(E) 5′ RACE for the THOR transcripts expressed by the lentiviral system. PCR agarose gel (left) confirms single band used in Sanger sequencing (right).

(F) 3′ RACE for the THOR transcripts expressed by the lentiviral system. PCR agarose gel (left) shows two bands utilized in Sanger sequencing (right).

(G) Coding probability scores for the transcripts were assessed by Coding Potential Assessment Tool (CPAT). NRAS and TP53 used as positive control, and SCHLAP1 as a negative control.

(H) Coding probability scores for the PhyloCSF and CPC tools for THOR and MYC. Values less than 0 suggest a lack of coding potential.

(I) Genome browser depiction of the THOR locus with aggregate ribosomal profiling track (red), aggregate poly-A RNA-seq track (green) and GENCODE v22 genome annotation obtained from the GWIPS-viz ribo-seq genome browser.

(J) H&E image of the testis and surrounding tissue architecture.

(K) H&E (left) and THOR ISH (right) for the human testis, rete, and adipose.