FIGURE

Fig. 2

ID
ZDB-FIG-180403-27
Publication
Lampe et al., 2017 - Francisella noatunensis subspecies noatunensis clpB deletion mutant impairs development of francisellosis in a zebrafish model
Other Figures
All Figure Page
Back to All Figure Page
Fig. 2

Intravascular injection experiment of zebrafish embryos with green fluorescent F.n.n. wt, F.n.n. ΔclpB and PBS suggests growth attenuation of the deletion mutant. (A) Injections of 102 –103 CFU were administered into the Duct of Cuvier (arrow) of zebrafish embryos (AB wt) and micrographs were made from the tail region (rectangle). (B) Micrographs from the tail region of zebrafish embryos 7 days post injection with green fluorescent F.n.n. gfp (wt), F.n.n. ΔclpB gfp (ΔclpB) and PBS. Only few weakly green fluorescent foci were visible in mutant infected embryos (arrowhead), 10x magnification. (C) Kaplan − Meier curve of cumulative survival of zebrafish embryos injected with F.n.n. gfp, F.n.n. ΔclpB gfp or PBS (control). The difference in cumulative survival between the mutant-injected and the wt-injected embryos was statistically significant (p = .0087). (D) Quantification of F.n.n. genome equivalents by qPCR on gDNA zebrafish embryos injected with F.n.n. gfp or F.n.n. ΔclpB gfp at 0 or 7 days post injection (dpi). Results are presented as mean ± SEM. Bacterial load was significantly higher in wt injected embryos 7 dpi (wt day 0 versus day 7, p = .0089 and ΔclpB day 0 versus day 7, p = .0175 and wt versus ΔclpB day 7, p = .0063). (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)

Expression Data

Expression Detail
Antibody Labeling
Phenotype Data

Phenotype Detail
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image.

Reprinted from Vaccine, 35(52), Lampe, E.O., Zingmark, C., Tandberg, J.I., Thrane, I.M.P., Brudal, E., Sjöstedt, A., Winther-Larsen, H.C., Francisella noatunensis subspecies noatunensis clpB deletion mutant impairs development of francisellosis in a zebrafish model, 7264-7272, Copyright (2017) with permission from Elsevier. Full text @ Vaccine