FIGURE

Fig. 2

ID

ZDB-FIG-180226-7

Publication

Casey et al., 2017 - Transplantation of Zebrafish Pediatric Brain Tumors into Immune-competent Hosts for Long-term Study of Tumor Cell Behavior and Drug Response

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Fig. 2

Tumor Engraftment and Progression. (A) Zebrafish primary CNS-PNET¹⁷ tumor cells labeled with mCherry were transplanted into the fourth ventricle of 2-dpf embryos. At 3 dpf, tumor cells are seen invading into surrounding brain tissue (arrows). At 8 dpf, tumor cells are growing in the ventricle; red tumor cells are also present in the surrounding brain tissue and kidneys (asterisks). At 28 dpf, tumors cells have invaded and grow throughout the host brain. (B) Top panel. Seven 3-dpf embryos transplanted with mCherry-labeled zebrafish brain tumor cells. Tumor cells can be transplanted consistently into hundreds of embryos, with high engraftment rates (i.e., typically 85/100 embryos). Middle and bottom panels. Three representative adult fish with transplanted tumors 4-8 weeks post-fertilization (wpf). Tumors can be visualized by fluorescence microscopy.

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Antibody Labeling

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