FIGURE

Fig. 4

ID

ZDB-FIG-171228-4

Publication

An et al., 2015 - Engineering FKBP-Based Destabilizing Domains to Build Sophisticated Protein Regulation Systems

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Fig. 4

Engineered FKBP** permits regulation of PTEN function in the zebrafish.

(A) The migration and growth of stable FKBP**-PTEN expressing U87MG cell clones injected into zebrafish embryos can be inhibited by Shld1. Prior to microinjection into the perivitelline cavity of 2 days post fertilization (dpf) zebrafish embryos, cells were pre-stained with DiI. Embryos were then transferred into fish water with (n = 11) or without (n = 13) Shld1 (4 μM) and maintained for 24h. Images were taken immediately after injections (0 days post injections, dpi) and after 24 h (1dpi). (B) The migration indices and the increase in tumor mass size of transplanted cells were quantified. ** p<0.01; ***p<0.001.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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